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| Product Name | NUR77 [ET1703-97] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Early response protein NAK1 antibody GFRP 1 antibody GFRP antibody GFRP1 antibody Growth factor inducible nuclear protein N10 antibody Growth Factor Inducible Nuclear Protein NP10 antibody Growth Factor Response Protein 1 antibody Hbr1 antibody HMR antibody Hormone Receptor antibody MGC9485 antibody N10 antibody N10 nuclear protein antibody NAK 1 antibody NAK1 antibody Nerve growth factor IB nuclear receptor variant 1 antibody NGFIB antibody NP 10 antibody NP10 antibody NR4A1 antibody NR4A1_HUMAN antibody Nuclear hormone receptor NUR/77 antibody Nuclear Hormone Receptor TR3 antibody Nuclear receptor subfamily 4 group A member 1 antibody NUR77 antibody NUR77, mouse, homolog of antibody Orphan nuclear receptor HMR antibody Orphan nuclear receptor NR4A1 antibody Orphan nuclear receptor TR3 antibody Orphan receptor tr3 antibody Receptor NGFIB antibody ST 59 antibody ST-59 antibody ST59 antibody Steroid receptor TR3 antibody Testicular receptor 3 antibody TR 3 antibody TR3 antibody TR3 orphan receptor antibody |
| Product description | Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encode orphan nuclear receptors which may comprise an additional subfamily within the nuclear receptor superfamily. The rat and human homologs of mouse Nurr1 are designated RNR1 and NOT, respectively. Both Nurr1 and Nur77 are growth factor inducible immediate early response genes. Induction of both Nurr1 and Nur77 is seen after membrane depolarization while only Nur77 induction is seen with NGF stimulation. JunD acts as a mediator for Nur77. An increase in Nur77 expression is seen in activated T cells during G0 to G1 transition and throughout the G1 phase. In addition to its function as an immediate early gene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, a potent immunosuppressant, has been shown to inhibit the ability of Nur77 to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirement of Nur77 for TCR-mediated apoptosis is still under debate. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Early response protein NAK1 antibody GFRP 1 antibody GFRP antibody GFRP1 antibody Growth factor inducible nuclear protein N10 antibody Growth Factor Inducible Nuclear Protein NP10 antibody Growth Factor Response Protein 1 antibody Hbr1 antibody HMR antibody Hormone Receptor antibody MGC9485 antibody N10 antibody N10 nuclear protein antibody NAK 1 antibody NAK1 antibody Nerve growth factor IB nuclear receptor variant 1 antibody NGFIB antibody NP 10 antibody NP10 antibody NR4A1 antibody NR4A1_HUMAN antibody Nuclear hormone receptor NUR/77 antibody Nuclear Hormone Receptor TR3 antibody Nuclear receptor subfamily 4 group A member 1 antibody NUR77 antibody NUR77 mouse homolog of antibody Orphan nuclear receptor HMR antibody Orphan nuclear receptor NR4A1 antibody Orphan nuclear receptor TR3 antibody Orphan receptor tr3 antibody Receptor NGFIB antibody ST 59 antibody ST-59 antibody ST59 antibody Steroid receptor TR3 antibody Testicular receptor 3 antibody TR 3 antibody TR3 antibody TR3 orphan receptor antibody |
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| Molecular Weight(MW) | 64 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus membrane. |
Application
Fig1: Western blot analysis of NUR77 on rat brain cells lysates using anti-NUR77 antibody at 1/500 dilution.
Application
Fig2: ICC staining NUR77 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NUR77 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining NUR77 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NUR77 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NUR77 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-NUR77 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NUR77 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse ovarian using anti-NUR77 antibody. Counter stained with hematoxylin.| Positive Control | Rat brain cells lysates, HepG2, NIH-3T3, Hela, human liver tissue, human colon cancer tissue, human breast cancer tissue, mouse testis tissue, mouse ovarian tissue. |
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| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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