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| Product Name | Cytokeratin 14 [A2C11] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | CK 14 antibody CK-14 antibody ck14 antibody Cytokeratin 14 antibody Cytokeratin-14 antibody Cytokeratin14 antibody Dowling Meara antibody EBS3 antibody EBS4 antibody Epidermolysis bullosa simplex antibody K14 antibody K1C14_HUMAN antibody Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody Keratin 14 antibody Keratin antibody Keratin type I cytoskeletal 14 antibody Keratin, type I cytoskeletal 14 antibody Keratin-14 antibody Keratin14 antibody Koebner antibody Krt 14 antibody Krt14 antibody NFJ antibody OTTHUMP00000164624 antibody type I cytoskeletal 14 antibody |
| Product description | Keratin 14 is a member of the type I keratin family of intermediate filament proteins. Keratin 14 is also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14). In humans it is encoded by the KRT14 gene. Keratin 14 is usually found as a heterodimer with type II keratin 5 and form the cytoskeleton of epithelial cells. Cytokeratin 14 is found in squamous epithelial basal cells, myoepithelium, some glandular epithelia, and mesothelial cells. Anti-Cytokeratin 14 is useful for distinguishing squamous cell carcinomas from other epithelial tumors, and for classifying metaplastic breast carcinomas. The Cytokeratin 14 (IHC555) antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of squamous cell carcinomas within the context of antibody panels, the patient’s clinical history and other diagnostic tests evaluated by a qualified pathologist. |
| Immunogen | Synthetic peptide within N terminal human Cytokeratin 14. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CK 14 antibody CK-14 antibody ck14 antibody Cytokeratin 14 antibody Cytokeratin-14 antibody Cytokeratin14 antibody Dowling Meara antibody EBS3 antibody EBS4 antibody Epidermolysis bullosa simplex antibody K14 antibody K1C14_HUMAN antibody Keratin 14 (epidermolysis bullosa simplex Dowling-Meara Koebner) antibody Keratin 14 antibody Keratin antibody Keratin type I cytoskeletal 14 antibody Keratin type I cytoskeletal 14 antibody Keratin-14 antibody Keratin14 antibody Koebner antibody Krt 14 antibody Krt14 antibody NFJ antibody OTTHUMP00000164624 antibody type I cytoskeletal 14 antibody |
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| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | P02533 |
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Application
Fig1: Western blot analysis of Cytokeratin 14 on A431 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Cytokeratin 14 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Flow cytometric analysis of Cytokeratin 14 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-32, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | A431 cell lysates, human skin tissue. |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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