| 存储条件 |
|---|
| Product Name | LAMP2 [D3-D8-D3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD107 antigen like family member B antibody CD107 antigen-like family member B antibody CD107b antibody LAMP 2 antibody LAMP 2C antibody LAMP-2 antibody LAMP2 antibody LAMP2_HUMAN antibody LAMPB antibody LGP 96 antibody LGP110 antibody LGP96 antibody Lysosomal associated membrane protein 2 antibody Lysosome associated membrane glycoprotein 2 antibody Lysosome associated membrane protein 2 antibody Lysosome-associated membrane glycoprotein 2 antibody Lysosome-associated membrane protein 2 antibody MAC3 antibody |
| Product description | Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation. Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens. |
| Immunogen | Recombinant protein within human LAMP2 aa 1-300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC-PFC |
| WB:1:500-1:2,000 ICC:1:50-1:100 IHC-P:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CD107 antigen like family member B antibody CD107 antigen-like family member B antibody CD107b antibody LAMP 2 antibody LAMP 2C antibody LAMP-2 antibody LAMP2 antibody LAMP2_HUMAN antibody LAMPB antibody LGP 96 antibody LGP110 antibody LGP96 antibody Lysosomal associated membrane protein 2 antibody Lysosome associated membrane glycoprotein 2 antibody Lysosome associated membrane protein 2 antibody Lysosome-associated membrane glycoprotein 2 antibody Lysosome-associated membrane protein 2 antibody MAC3 antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 45 kDa |
| Cellular Localization | Cell membrane, lysosome membrane, endosome membrane, autophagosome membrane. |
| SwissProt ID | P13473 |
|---|
Application
Fig1: Western blot analysis of LAMP2 on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining of LAMP2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-62, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig3: ICC staining of LAMP2 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-62, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-LAMP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-62, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-LAMP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-62, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LAMP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-62, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig7: Flow cytometric analysis of LAMP2 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-62, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | SiHa cell lysates, A549 cell, HT-29 cell, human liver carcinoma tissue, human prostate tissue, human placenta tissue, SiHa. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:100 IHC-P:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
