| 存储条件 |
|---|
| Product Name | FKBP12 [JM11-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases which bind to and mediate the effects of immunosuppressive drugs such as Cyclosporin, FK506 and Rapamycin. FKBP12.6, also known as FK506-binding protein 1B, is a 108 amino acid immunophilin belonging to the FKBP-type PPIase family. Subcellularly localized to the cytoplasm, FKBP12.6 binds to RyR in cardiac muscle sarcoplasmic reticulum and possibly plays a unique physiological role in excitation-contraction coupling in cardiac muscle. FKBP12.6 also catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Ubiquitously expressed, FKBP12.6 is found at highest levels in brain and thymus. FKBP12.6 is expressed as two isoforms produced by alternative splicing. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF |
| WB:1:500-1:1,000 ICC:1:100-1:500 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | 12 kDa FK506-binding protein antibody 12 kDa FKBP antibody Calstabin 1 antibody FK506 binding protein 1 antibody FK506 binding protein 12 antibody FK506 binding protein 1A (12kD) antibody FK506 binding protein 1A 12kDa antibody FK506 binding protein 1A antibody FK506 binding protein T cell 12 kD antibody FK506 binding protein T cell 12 kD antibody FK506 binding protein12 antibody FK506-binding protein 1A antibody FKB1A_HUMAN antibody FKBP 12 antibody FKBP 1A antibody FKBP-12 antibody FKBP-1A antibody FKBP1 antibody FKBP12 antibody FKBP12 Exip3 antibody FKBP12C antibody fkbp1a antibody Immunophilin FKBP12 antibody Peptidyl prolyl cis trans isomerase antibody Peptidyl-prolyl cis-trans isomerase FKBP1A antibody PKC12 antibody PKCI2 antibody PPIase antibody PPIase FKBP1A antibody Protein kinase C inhibitor 2 antibody Rotamase antibody |
|---|---|
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of FKBP12 on different cells lysates using anti-FKBP12 antibody at 1/500 dilution. Positive control: Line 1: SH-SY5Y Line 2:V937
Application
Fig2: ICC staining FKBP12 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining FKBP12 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining FKBP12 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | SH-SY5Y, V937, HepG2, A431, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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