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| Product Name | Cytokeratin 17 Mouse Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair (By similarity). Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state (By similarity). Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway (By similarity). Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors (By similarity). May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. |
| Immunogen | Synthetic peptide within Human Cytokeratin 17 aa 383-432 / 432. |
| Clonality | monoclonal |
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| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Protein G |
| Alternative Names | 39.1 antibody CK 17 antibody CK-17 antibody Cytokeratin-17 antibody K17 antibody K1C17_HUMAN antibody Keratin 17 antibody keratin 17 epitope S1 antibody keratin 17 epitope S2 antibody keratin 17 epitope S4 antibody Keratin 17 type I antibody Keratin antibody Keratin type I cytoskeletal 17 antibody keratin type i cytoskeletal 17 [version 1] antibody Keratin-17 antibody KRT17 antibody PC antibody PC2 antibody PCHC1 antibody type I cytoskeletal 17 antibody |
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| Molecular Weight(MW) | 48kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q04695 |
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WB
Western blot analysis of Cytokeratin 17 on different lysates with Mouse anti-Cytokeratin 17 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: MCF7 cell lysate (negative), Lane 4: HepG2 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-Cytokeratin 17 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Cytokeratin 17 with Mouse anti-Cytokeratin 17 antibody at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cytokeratin 17 antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of Cytokeratin 17 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | SiHa cell lysates, human liver carcinoma tissue, human skin tissue. |
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| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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