This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
Immunogen
Purified recombinant fragment of human NFKB2 (AA: 712-900) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WB,FC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human,Mouse
Concentration
1mg/mL
Purification
Unpurified
Target Information
Alternative Names
CVID10 antibody DNA binding factor KBF2 antibody H2TF1 antibody Lymphocyte translocation chromosome 10 protein antibody LYT 10 antibody NF kB2 antibody NFKB p52/p100 subunit antibody Nuclear factor Kappa B subunit 2 antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody Oncogene Lyt 10 antibody p100 antibody Transcription factor NFKB2 antibody
Western blot analysis of NFKB2 against human NFKB2 (AA: 712-900) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of NFKB2 against HEK293 (1) and NFKB2 (AA: 712-900)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of NFKB2 against NIH/3T3 (1), MCF-7 (2), and THP-1 (3) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Positive Control
NIH/3T3, MCF-7, and THP-1 cell lysate
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
