| 存储条件 |
|---|
| Product Name | Alpha Actinin 2 [JF0980] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,IHC,IP |
| WB:1:1,000-1:2,000 IHC:1:50-1:200 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Molecular Weight(MW) | 104 kDa |
|---|---|
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of ACTN2 on human heart lysates using anti-ACTN2 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.| Positive Control | Human heart tissue, rat skeletal muscle tissue, rat heart tissue, mouse skeletal tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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