| 存储条件 |
|---|
| Product Name | VAMP8 [JF0963] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EDB antibody Endobrevin antibody VAMP 8 antibody VAMP-8 antibody VAMP8 antibody VAMP8_HUMAN antibody Vesicle associated membrane protein 8 antibody Vesicle-associated membrane protein 8 antibody |
| Product description | Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAPs (soluble NSF attachment proteins) and Synaptotagmin. Endobrevin, also designated VAMP-8 or ED, is a 100 amino acid single-pass type IV membrane protein that belongs to the synaptobrevin family. Similar in sequence to the synaptobrevins, endobrevin is abundantly expressed in kidney, moderately expressed in heart and spleen, and slightly expressed in brain, thymus and liver. Endobrevin interacts specifically with the SNAPs, most likely through an endobrevin-containing SNARE complex. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | EDB antibody Endobrevin antibody VAMP 8 antibody VAMP-8 antibody VAMP8 antibody VAMP8_HUMAN antibody Vesicle associated membrane protein 8 antibody Vesicle-associated membrane protein 8 antibody |
|---|---|
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Lysosome membrane, Early endosome membrane, Late endosome membrane, Cell membrane. |
Application
Fig1: Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse kidney
Application
Fig2: ICC staining VAMP8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining VAMP8 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining VAMP8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with VAMP8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SW480, 293T, Hela, mouse brain tissue, mouse kidney tissue, human kidney tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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