| 存储条件 |
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| Product Name | MMP13 [JF0893] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLG 3 antibody CLG3 antibody Collagenase 3 antibody Collagenase3 antibody MANDP1 antibody Matrix metallopeptidase 13 (collagenase 3) antibody Matrix Metalloproteinase 13 antibody Matrix metalloproteinase-13 antibody MMP 13 antibody MMP-13 antibody Mmp13 antibody MMP13_HUMAN antibody |
| Product description | The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-13 (also designated collagenase-3) is produced by breast carcinomas and degrades collagen types I, II and III. MMP-13 has wide substrate specificity, and its physiologic expression is limited to situations in which rapid and effective remodeling of collagenous ECM takes place, such as fetal bone development and adult bone remodeling. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CLG 3 antibody CLG3 antibody Collagenase 3 antibody Collagenase3 antibody MANDP1 antibody Matrix metallopeptidase 13 (collagenase 3) antibody Matrix Metalloproteinase 13 antibody Matrix metalloproteinase-13 antibody MMP 13 antibody MMP-13 antibody Mmp13 antibody MMP13_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of MMP13 on MCF-7 cells lysates using anti-MMP13 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining MMP13 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MMP13 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MMP13 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MMP13 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MMP13 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP13 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with MMP13 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, Hela, SW480, human breast carcinoma tissue, human lung cancer tissue, human breast tissue. |
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| Application Notes | WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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