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| Product Name | LTBR [1D1C5C8] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | CD18 antibody D12S370 antibody LT beta R antibody LTBETAR antibody Ltbr antibody Lymphotoxin B receptor antibody Lymphotoxin beta receptor (TNFR superfamily, member 3) antibody Lymphotoxin beta receptor antibody Lymphotoxin-beta receptor antibody TNF R III antibody TNF-RIII antibody TNFCR antibody TNFR RP antibody TNFR superfamily member 3 antibody TNFR-III antibody TNFR2 RP antibody TNFR2RP antibody TNFR3 antibody TNFRII antibody TNFRRP antibody TNFRSF 3 antibody TNFRSF3 antibody TNR3_HUMAN antibody Tumor necrosis factor C receptor antibody Tumor necrosis factor receptor 2 related protein antibody Tumor necrosis factor receptor 2-related protein antibody Tumor necrosis factor receptor superfamily member 3 antibody Tumor necrosis factor receptor superfamily member 3 precursor antibody Tumor necrosis factor receptor type III antibody |
| Product description | This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed. |
| Immunogen | Purified recombinant fragment of human LTBR (AA: extra 31-227) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/mL |
| Alternative Names | CD18 antibody D12S370 antibody LT beta R antibody LTBETAR antibody Ltbr antibody Lymphotoxin B receptor antibody Lymphotoxin beta receptor (TNFR superfamily member 3) antibody Lymphotoxin beta receptor antibody Lymphotoxin-beta receptor antibody TNF R III antibody TNF-RIII antibody TNFCR antibody TNFR RP antibody TNFR superfamily member 3 antibody TNFR-III antibody TNFR2 RP antibody TNFR2RP antibody TNFR3 antibody TNFRII antibody TNFRRP antibody TNFRSF 3 antibody TNFRSF3 antibody TNR3_HUMAN antibody Tumor necrosis factor C receptor antibody Tumor necrosis factor receptor 2 related protein antibody Tumor necrosis factor receptor 2-related protein antibody Tumor necrosis factor receptor superfamily member 3 antibody Tumor necrosis factor receptor superfamily member 3 precursor antibody Tumor necrosis factor receptor type III antibody |
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| Molecular Weight(MW) | 46.7kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P36941 |
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Application
Western blot analysis of LTBR against human LTBR (AA: extra 31-227) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-57 against HEK293 (1) and LTBR (AA: extra 31-227)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-57 against Hela (1), NIH/3T3 (2), and HEK293 (3) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of LTBR was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-57, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela, NIH/3T3, and HEK293 cell HL-60 cells |
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| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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