ADCAD2 antibody C030016K15Rik antibody Cd antibody FLJ90062 antibody FLJ90421 antibody LDL receptor related protein 6 antibody Low density lipoprotein receptor related protein 6 antibody Low-density lipoprotein receptor-related protein 6 antibody LRP-6 antibody LRP6 antibody LRP6_HUMAN antibody OTTHUMP00000238979 antibody OTTHUMP00000238980 antibody OTTHUMP00000238982 antibody STHAG7 antibody
Product description
This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.
Immunogen
Purified recombinant fragment of human LRP6 (AA: extra 1198-1370) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
ADCAD2 antibody C030016K15Rik antibody Cd antibody FLJ90062 antibody FLJ90421 antibody LDL receptor related protein 6 antibody Low density lipoprotein receptor related protein 6 antibody Low-density lipoprotein receptor-related protein 6 antibody LRP-6 antibody LRP6 antibody LRP6_HUMAN antibody OTTHUMP00000238979 antibody OTTHUMP00000238980 antibody OTTHUMP00000238982 antibody STHAG7 antibody
Molecular Weight(MW)
180kDa
Cellular Localization
Membrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD.
Western blot analysis of LRP6 against human LRP6 (AA: extra 1198-1370) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-56 against HEK293 (1) and LRP6 (AA: extra 1198-1370)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of LRP6 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-56, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Positive Control
HL-60 cells
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
