AI848829 antibody FLJ12929 antibody Low density lipoprotein receptor related protein 12 antibody Low-density lipoprotein receptor-related protein 12 antibody LRP 12 antibody LRP-12 antibody LRP12 antibody LRP12_HUMAN antibody ST7 antibody Suppressor of tumorigenicity 7 protein antibody
Product description
This gene encodes a member of the low-density lipoprotein receptor related protein family. The product of this gene is a transmembrane protein that is differentially expressed in many cancer cells. Alternate splicing results in multiple transcript variants.
Immunogen
Purified recombinant fragment of human LRP12 (AA: extra 355-492) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG2b
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
AI848829 antibody FLJ12929 antibody Low density lipoprotein receptor related protein 12 antibody Low-density lipoprotein receptor-related protein 12 antibody LRP 12 antibody LRP-12 antibody LRP12 antibody LRP12_HUMAN antibody ST7 antibody Suppressor of tumorigenicity 7 protein antibody
Western blot analysis of LRP12 against human LRP12 (AA: extra 355-492) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of LRP12 against HEK293 (1) and LRP12 (AA: extra 355-492)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of LRP12 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-60, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Positive Control
HL-60 cells
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
