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| Product Name | FGFR3 [JM81-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACH antibody CD 333 antibody CD333 antibody CD333 antigen antibody CEK 2 antibody CEK2 antibody FGFR 3 antibody FGFR-3 antibody FGFR3 antibody FGFR3_HUMAN antibody Fibroblast growth factor receptor 3 (achondroplasia thanatophoric dwarfism) antibody Fibroblast growth factor receptor 3 antibody Heparin binding growth factor receptor antibody HSFGFR3EX antibody Hydroxyaryl protein kinase antibody JTK 4 antibody JTK4 antibody MFR 3 antibody SAM 3 antibody Tyrosine kinase JTK 4 antibody Tyrosine kinase JTK4 antibody Z FGFR 3 antibody |
| Product description | Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Like other growth factors, FGFs act by binding and activating specific cell surface receptors. These include the Flg receptor or FGFR-1, the Bek receptor or FGFR-2, FGFR-3, FGFR-4, FGFR-5 and FGFR-6. These receptors usually contain an extracellular ligand-binding region containing three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The gene encoding human FGFR-3 maps to chromosome 4p16 and is alternatively spliced to produce three isoforms that are expressed in brain, kidney and testis. Defects in FGFR-3 are associated with several diseases, including Crouzon syndrome, achondroplasia, thanatophoric dysplasia, craniosynostosis adelaide type and hypochondroplasia. Mutations in FGFR-3 are also a cause of some bladder and cervical cancers. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1：50-1：100: | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ACH antibody CD 333 antibody CD333 antibody CD333 antigen antibody CEK 2 antibody CEK2 antibody FGFR 3 antibody FGFR-3 antibody FGFR3 antibody FGFR3_HUMAN antibody Fibroblast growth factor receptor 3 (achondroplasia thanatophoric dwarfism) antibody Fibroblast growth factor receptor 3 antibody Heparin binding growth factor receptor antibody HSFGFR3EX antibody Hydroxyaryl protein kinase antibody JTK 4 antibody JTK4 antibody MFR 3 antibody SAM 3 antibody Tyrosine kinase JTK 4 antibody Tyrosine kinase JTK4 antibody Z FGFR 3 antibody |
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| Molecular Weight(MW) | 100 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P22607 |
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Application
Fig1: Western blot analysis of FGFR3 on Hela cells lysates using anti-FGFR3 antibody at 1/500 dilution.
Application
Fig2: ICC staining FGFR3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining FGFR3 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining FGFR3 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HepG2 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, MCF-7, SH-SY5Y, HepG2, human breast cancer, human kidney tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1：50-1：100: |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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