| 存储条件 |
|---|
| Product Name | Dynamin 1 [A1B2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Immunogen | Recombinant protein within human Dynamin 1 aa 500-800. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,IHC-P,FC |
| WB:1:500-1:2,000IHC-P:1:50-1:200FC:1:50-1:100 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | B dynamin antibody D100 antibody DNM 1 antibody DNM antibody DNM1 antibody DYN1_HUMAN antibody Dynamin antibody Dynamin-1 antibody Dynamin1 antibody |
|---|---|
| Molecular Weight(MW) | 97 kDa |
| Cellular Localization | Cytoskeleton, cytoplasm. |
Application
Fig1: Western blot analysis of Dynamin 1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Dynamin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Dynamin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Flow cytometric analysis of Dynamin 1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-42, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | PC-3M cell lysates, rat brain tissue, mouse brain tissue, A549. |
|---|---|
| Application Notes | WB:1:500-1:2,000IHC-P:1:50-1:200FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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