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| Product Name | PDHA1 [JF996-0] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ODPA_HUMAN antibody PDH antibody PDHA antibody PDHA1 antibody PDHCE1A antibody PDHE1 A type I antibody PDHE1-A type I antibody PHE1A antibody Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody Pyruvate Dehydrogenase E1 alpha antibody Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody |
| Product description | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial matrix enzyme complex that functions as the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The E1 enzyme of the PDH complex is made up of a heterotetramer of two α and two β subunits. The E1-α subunit (PDH-E1α) contains the E1 active site and plays a key role in the function of the PDH complex. The PDH complex is regulated by phosphorylation and dephosphorylation of PDH-E1α. The gene encoding for PDH-E1α maps to chromosome Xp22.12, and a 20bp deletion in the last exon of this gene is sufficient to cause PDH deficiency, which causes a broad range of symptoms including the development of seizures, mental retardation and spasticity, as well as intermittent episodes of lactic acidosis associated with cerebellar ataxia. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ODPA_HUMAN antibody PDH antibody PDHA antibody PDHA1 antibody PDHCE1A antibody PDHE1 A type I antibody PDHE1-A type I antibody PHE1A antibody Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody Pyruvate dehydrogenase complex E1 alpha polypeptide 1 antibody Pyruvate Dehydrogenase E1 alpha antibody Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial antibody |
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| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Mitochondrion matrix. |
Application
Fig1: Western blot analysis of PDHA1 on different lysates using anti-PDHA1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: A431 Lane 3: Mouse heart
Application
Fig2: ICC staining PDHA1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PDHA1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PDHA1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Application
Fig11: Flow cytometric analysis of Hela cells with PDHA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | SH-SY-5Y, A431, Hela, HepG2, 293T, human lung cancer tissue, human breast carcinoma tissue, mouse heart tissue, mouse colon tissue, mouse stomach tissue, human kidney tissue, mouse skeletal muscle tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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