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| Product Name | Argonaute 2 [JF0992] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Ago 2 antibody AGO2_HUMAN antibody Argonaute 2 antibody argonaute 2, RISC catalytic component antibody Argonaute RISC catalytic component 2 antibody Argonaute2 antibody CTA-204B4.6 antibody dAgo2 antibody eIF 2C 2 antibody eIF-2C 2 antibody eIF2C 2 antibody Eif2c2 antibody Eukaryotic translation initiation factor 2C 2 antibody Eukaryotic translation initiation factor 2C subunit 2 antibody hAgo2 antibody MGC3183 antibody PAZ Piwi domain protein antibody PPD antibody Protein argonaute-2 antibody Protein slicer antibody Q10 antibody Slicer protein antibody |
| Product description | Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5). |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Ago 2 antibody AGO2_HUMAN antibody Argonaute 2 antibody argonaute 2 RISC catalytic component antibody Argonaute RISC catalytic component 2 antibody Argonaute2 antibody CTA-204B4.6 antibody dAgo2 antibody eIF 2C 2 antibody eIF-2C 2 antibody eIF2C 2 antibody Eif2c2 antibody Eukaryotic translation initiation factor 2C 2 antibody Eukaryotic translation initiation factor 2C subunit 2 antibody hAgo2 antibody MGC3183 antibody PAZ Piwi domain protein antibody PPD antibody Protein argonaute-2 antibody Protein slicer antibody Q10 antibody Slicer protein antibody |
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| Molecular Weight(MW) | 97 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Argonaute 2 on different lysates using anti-Argonaute 2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7
Application
Fig2: ICC staining Argonaute 2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Argonaute 2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with Argonaute 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | AGS, MCF-7, Hela, human kidney tissue, mouse kidney tissue, mouse stomach tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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