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| Product Name | Vitamin D Binding protein [JM10-36] |
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| Antibody Type | Primary Antibodies |
| Product description | Vitamin D-binding protein (DBP) is a multi-functional serum protein that binds to the plasma membranes of numerous cell types and mediates a variety of cellular functions. The locus of the DBP protein (also known as group-specific component protein or GC) is located at human chromosome 4q13.3. DBP functions in organ-specific transportation of vitamin D and its metabolites to the various target organs of the vitamin D endocrine system. In addition, DBP has immunomodulatory properties and is able to bind to the surface of leukocytes. DBP binds to the plasma membrane through a chondroitin sulfate proteoglycan. DBP serves as a co-chemotactic factor for C5a to enhance the chemotactic activity of C5a. DBP can also bind to globular Actin with high affinity and is involved in the clearance of Actin from the blood. DBP plays an important role in osteoclast differentiation. The diverse cellular functions of DBP require its cell surface binding ability to mediate different biological processes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC,FC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | DBP antibody DBP/GC antibody GC antibody Gc globulin antibody Gc-globulin antibody GRD3 antibody Group specific component antibody Group specific component vitamin D binding protein antibody Group-specific component antibody hDBP antibody VDB antibody VDBG antibody VDBP antibody Vitamin D binding alpha globulin antibody Vitamin D-binding protein antibody VTDB_HUMAN antibody |
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| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P02774 |
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Application
Fig1: Western blot analysis of DBP on human lung lysates using anti- DBP antibody at 1/1,000 dilution.
Application
Fig2: ICC staining DBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining DBP in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining DBP in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- DBP antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HepG2 cells with DBP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SKOV-3, Hela, HepG2, human kidney tissue, human lung tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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