The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway.
Immunogen
Purified recombinant fragment of human TRADD (AA: 1-177) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WB,FC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human
Concentration
1mg/mL
Purification
Unpurified
Target Information
Alternative Names
9130005N23Rik antibody AA930854 antibody TNFR1 associated DEATH domain protein antibody TNFR1-associated DEATH domain protein antibody TNFRSF1A associated via death domain antibody TNFRSF1A-associated via death domain antibody tradd antibody TRADD_HUMAN antibody Tumor necrosis factor receptor type 1 associated DEATH domain protein antibody Tumor necrosis factor receptor type 1-associated DEATH domain protein antibody
Western blot analysis of TRADD against human TRADD (AA: 1-177) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of TRADD against HEK293 (1) and TRADD (AA: 1-177)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of TRADD was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-24, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Positive Control
Hela cells
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
