| 存储条件 |
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| Product Name | Thymidine Kinase 1 [JF0970] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cytosolic antibody KITH_HUMAN antibody Thymidine kinase 1 antibody Thymidine kinase 1 soluble antibody Thymidine kinase 1 soluble isoform antibody Thymidine kinase antibody Thymidine kinase cytosolic antibody TK 1 antibody TK 2 antibody TK1 antibody Tk1a antibody Tk1b antibody TK2 antibody |
| Product description | Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division. Because of this, Thymidine Kinase can be used as a proliferation marker in the diagnosis, treatment, and follow-up of malignant diseases, especially hematological malignancies. Thymidine Kinase may be observed as a monomer, dimer, trimer or tetramer. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | cytosolic antibody KITH_HUMAN antibody Thymidine kinase 1 antibody Thymidine kinase 1 soluble antibody Thymidine kinase 1 soluble isoform antibody Thymidine kinase antibody Thymidine kinase cytosolic antibody TK 1 antibody TK 2 antibody TK1 antibody Tk1a antibody Tk1b antibody TK2 antibody |
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| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P04183 |
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Application
Fig1: Western blot analysis of Thymidine Kinase 1 on different lysates using anti-Thymidine Kinase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela
Application
Fig2: ICC staining Thymidine Kinase 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Thymidine Kinase 1 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Thymidine Kinase 1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Thymidine Kinase 1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with Thymidine Kinase 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | NIH/3T3, SW480, Hela, 293T, human tonsil tissue. |
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| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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