KIAA0816 antibody LDLR dan antibody Low density lipoprotein receptor related protein 4 antibody Low-density lipoprotein receptor-related protein 4 antibody LRP-4 antibody LRP10 antibody Lrp4 antibody LRP4_HUMAN antibody MEGF7 antibody Multiple epidermal growth factor like domains 7 antibody Multiple epidermal growth factor-like domains 7 antibody
Product description
Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. May play an essential role in the process of digit differentiation.
Immunogen
Purified recombinant fragment of human LRP4 (AA: extra 21-189) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
KIAA0816 antibody LDLR dan antibody Low density lipoprotein receptor related protein 4 antibody Low-density lipoprotein receptor-related protein 4 antibody LRP-4 antibody LRP10 antibody Lrp4 antibody LRP4_HUMAN antibody MEGF7 antibody Multiple epidermal growth factor like domains 7 antibody Multiple epidermal growth factor-like domains 7 antibody
Western blot analysis of LRP4 against human LRP4 (AA: extra 21-189) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of LRP4 against HEK293 (1) and LRP4 (AA: extra 21-189)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Positive Control
Expressed in bone; present in osteoblasts and osteocytes.
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
