| 存储条件 | -20℃ |
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Mouse G-CSF ELISA Kit (CSF3) is a Sandwich (quantitative) ELISA for the measurement of Mouse G-CSF (CSF3) in Mouse in Biofluids, Cell Culture Media samples.
隐藏别名
Csfg, Csf3, Granulocyte colony-stimulating factor, G-CSF
Colorimetric
Plasma, Cell culture supernatant, Serum
Mouse
Sandwich (quantitative)
< 0.5 pg/mL
0.62 - 150 pg/mL
Microplate
Abcam's G-CSF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse G-CSF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse G-CSF coated on a 96- well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100684
Representative Standard Curve using ab100684
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