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Mouse Fractalkine ELISA Kit (CX3CL1) is a Sandwich (quantitative) ELISA for the measurement of Mouse Fractalkine (CX3CL1) in Mouse in Biofluids, Cell Culture Media samples.
隐藏别名
Cx3c, Fkn, Scyd1, Cx3cl1, Fractalkine, FK, ABCD-3, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1
Colorimetric
Plasma, Cell culture supernatant, Serum
Mouse
Sandwich (quantitative)
< 0.3 ng/mL
0.274 - 200 ng/mL
Microplate
Abcam's CX3CL1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CX3CL1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse CX3CL1 coated on a 96- well plate. Standards and samples are pipetted into the wells and CX3CL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CX3CL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CX3CL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The standard is affinity purified.
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Representative standard curve using ab100683
Representative standard curve using ab100683
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