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Mouse ICAM1 ELISA Kit (CD54) is a Sandwich (quantitative) ELISA for the measurement of Mouse ICAM1 (CD54) in Mouse in Biofluids, Cell Culture Media samples.
隐藏别名
CD54, Icam-1, Icam1, Intercellular adhesion molecule 1, ICAM-1, MALA-2, MyD10
Colorimetric
Plasma, Cell culture supernatant, Serum
Mouse
Sandwich (quantitative)
< 20 pg/mL
24.69 - 6000 pg/mL
Microplate
Abcam's ICAM1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse ICAM1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse ICAM1 coated on a 96- well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve
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