| 存储条件 |
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| Product Name | Chromogranin-A(C-term) [D11-2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | beta Granin antibody betagranin (N-terminal fragment of chromogranin A) antibody catestatin antibody CgA antibody CHG A antibody Chga antibody chromofungin antibody Chromogranin A antibody Chromogranin A parathyroid secretory protein 1 antibody Chromogranin A precursor antibody ChromograninA antibody CMGA_HUMAN antibody ER-37 antibody Pancreastatin antibody Parastatin antibody Parathyroid secretory protein 1 antibody Pituitary secretory protein I antibody Secretory protein I antibody SP I antibody SP-I antibody SP1 antibody SPI antibody Vasostatin antibody Vasostatin I antibody Vasostatin II antibody |
| Product description | Chromogranins (secretogranins) are acidic glycoproteins that localize within secretory granules of endocrine, neuroendocrine and neuronal tissue. Family members include chromogranin A (Chr-A), chromogranin B (Chr-B, also known as secretogranin I) chromogranin C (also known as secretogranin II or Sg II), secretogranin III (Sg III or SCG3). High levels of Chr-A expression is a characteristic of neuroendocrine tumors. Pancreastatin is a peptide derived from Chr-A which inhibits insulin secretion, exocrine pancreatic secretion and gastric acid secretion. Pancreastatin exists as two forms; the major form is expressed in stomach and colon extracts. In neuroendocrine cells the level Sg II has been shown to increase four-fold in response to histamine, while levels of Chr-A and Chr-B showed little or no increase. Sg III is an acidic secretory protein expressed in neuronal and endocrine cells. In the anterior lobe of the rat pituitary gland, Sg III is present in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, though not in somatotropes. Sg III and carboxypeptidase E (CPE) bind specifically to cholesterol-rich secretory granule (SG) membranes. |
| Immunogen | Peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCICC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | beta Granin antibody betagranin (N-terminal fragment of chromogranin A) antibody catestatin antibody CgA antibody CHG A antibody Chga antibody chromofungin antibody Chromogranin A antibody Chromogranin A parathyroid secretory protein 1 antibody Chromogranin A precursor antibody ChromograninA antibody CMGA_HUMAN antibody ER-37 antibody Pancreastatin antibody Parastatin antibody Parathyroid secretory protein 1 antibody Pituitary secretory protein I antibody Secretory protein I antibody SP I antibody SP-I antibody SP1 antibody SPI antibody Vasostatin antibody Vasostatin I antibody Vasostatin II antibody |
|---|---|
| Molecular Weight(MW) | 86 kDa |
| Cellular Localization | Secreted. Secretory vesicle lumen. |
Application
Fig1: Western blot analysis of Chromogranin A on PC-12 using anti-Chromogranin A antibody at 1/500 dilution.
Application
Fig2: ICC staining Chromogranin A(red) in Hela cells. The nuclear counter stain is DAPI(blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Chromogranin A(red) in LOVO cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Chromogranin A(red) in PANC-1 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.| Positive Control | PC-12, Hela, LOVO, PANC-1, rat small intestine tissue, rat pancreas tissue, mouse pancreas tissue. |
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| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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