| 存储条件 |
|---|
| Product Name | Beta-Catenin [A6-F8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Beta catenin antibody Beta-catenin antibody Cadherin associated protein antibody Catenin (cadherin associated protein), beta 1, 88kDa antibody Catenin beta 1 antibody Catenin beta-1 antibody CATNB antibody CHBCAT antibody CTNB1_HUMAN antibody CTNNB antibody CTNNB1 antibody DKFZp686D02253 antibody FLJ25606 antibody FLJ37923 antibody OTTHUMP00000162082 antibody OTTHUMP00000165222 antibody OTTHUMP00000165223 antibody OTTHUMP00000209288 antibody OTTHUMP00000209289 antibody |
| Product description | β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. |
| Immunogen | Synthetic peptide (KLH-coupled) within human Beta-catenin aa 320-400. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Beta catenin antibody Beta-catenin antibody Cadherin associated protein antibody Catenin (cadherin associated protein) beta 1 88kDa antibody Catenin beta 1 antibody Catenin beta-1 antibody CATNB antibody CHBCAT antibody CTNB1_HUMAN antibody CTNNB antibody CTNNB1 antibody DKFZp686D02253 antibody FLJ25606 antibody FLJ37923 antibody OTTHUMP00000162082 antibody OTTHUMP00000165222 antibody OTTHUMP00000165223 antibody OTTHUMP00000209288 antibody OTTHUMP00000209289 antibody |
|---|---|
| Molecular Weight(MW) | 85kDa |
| Cellular Localization | Cytoplasm, Nucleus |
Application
Fig1: Western blot analysis of beta-catenin on different cell lysates using anti- beta-catenin antibody at 1/2000 dilution.
Application
Fig2: ICC staining Beta-Catenin in NCCIT cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with Beta-catenin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.| Positive Control | A431, NCCIT, SW480, HT-29, mouse brain, Hela, rat testis tissue, human liver tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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