Rabbit polyclonal antibody to Phospho-LRP6 (Ser1420)
Antibody Type
Primary Antibodies
Immunogen
A synthesized peptide derived from human LRP6 around the phosphorylation site of Ser1420.
Modification
p-Ser1420
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IF
IHC:1:100-1:500
Species Reactivity
HumanMouse
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Symbol
LRP6
Gene Synonyms
ADCAD2 STHAG7
Gene Full Name
LDL receptor related protein 6
Gene Summary
This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]
Molecular Weight(MW)
180kDa
Cellular Localization
Cell membrane>Single-pass type I membrane protein. Endoplasmic reticulum. Membrane raft.
Application
IHC
Phospho-LRP6 (Ser1420) Antibody staining Hela cells(heat shock treatment) by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).
Application Notes
IHC:1:100-1:500
Additional Information
Form
Liquid
Storage Instructions
Store at -20 °C. Stable for 12 months from date of receipt.
Storage Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
