| 存储条件 |
|---|
| Product Name | IDO |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 3-dioxygenase antibody I23O1_HUMAN antibody IDO 1 antibody IDO antibody IDO-1 antibody IDO1 antibody INDO antibody indolamine 2,3 dioxygenase antibody Indole 2 3 dioxygenase antibody indoleamine 2 3 dioxygenase 1 antibody indoleamine 2 3 dioxygenase antibody Indoleamine 2,3-dioxygenase 1 antibody Indoleamine pyrrole 2 3 dioxygenase antibody Indoleamine-pyrrole 2 antibody |
| Product description | DescriptionIndoleamine 2,3-dioxygenase (IDO) is an IFN-? inducible enzyme that catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. The gene encoding human IDO maps to chromosome 8p12-p11. IDO, also known as INDO, is an important modulator of immunological responses and protects allogeneic concepti from alloreactive maternal lymphocytes. IDO mediates an interesting inhibitory effect of HeLa cells co-cultured with human PBLs. The ILN-2-induced proliferation response of PBLs is diminished in the presence of HeLa cells while an IDO inhbitor negates this effect. Flow cytometric analysis indicates both mature and immature CD123-positive dentritic cells suppress T cell activity using IDO. IDO-transfected cells co-cultured with T cells reduces T cell proliferation. Additionally, adopted transfer of donor T cells reduces donor T cell numbers in IDO-transgenic mice. The pharmacological or genetic manipulation of IDO may be useful for supressing undesirable T cell response. |
| Immunogen | Recombinant full length IDO of mouse origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Rat |
| Tested Applications | IFIHC-PIPWB |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | 3-dioxygenase antibody I23O1_HUMAN antibody IDO 1 antibody IDO antibody IDO-1 antibody IDO1 antibody INDO antibody indolamine 2 3 dioxygenase antibody Indole 2 3 dioxygenase antibody indoleamine 2 3 dioxygenase 1 antibody indoleamine 2 3 dioxygenase antibody Indoleamine 2 3-dioxygenase 1 antibody Indoleamine pyrrole 2 3 dioxygenase antibody Indoleamine-pyrrole 2 antibody |
|---|---|
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P14902 |
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Western blot
Fig1: Western blot analysis of IDO expression in non-transfected (A) and mouse IDO transfected (B) 293T whole cell lysates.
Immunoperoxidase
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of nongerminal center cells.| Positive Control | 293T, human lymph node tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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