| 存储条件 |
|---|
| Product Name | EGFR mutant |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERBB; HER1; mENA; ERBB1; PIG61 |
| Immunogen | Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Alternative Names | ERBB HER1 mENA ERBB1 PIG61 |
|---|---|
| Molecular Weight(MW) | 175kDa |
| Entrez Gene | 1956 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using EGFR mutant mAb against human EGFR mutant recombinant protein. (Expected MW is 36.9 kDa)
Application
Figure 2: Western blot analysis using EGFR mutant mAb against HEK293 (1) and EGFR mutant (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using EGFR mutant mouse mAb against SPC-A-1 (1), A549 (2), HepG2 (3) and MCF-7 (4) cell lysate.
Application
Figure 4: Flow cytometric analysis of HepG2 cells using EGFR mutant mouse mAb (green) and negative control (purple).
Application
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EGFR mutant mouse mAb with DAB staining.
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EGFR mutant mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
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