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| Product Name | DFNA5/GSDME [A1H3] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | 2310037D07Rik antibody 4932441K13Rik antibody Deafness, autosomal dominant 5 antibody Deafness, autosomal dominant 5 protein antibody DFNA5 antibody DFNA5 gene antibody DFNA5_HUMAN antibody Dfna5h antibody EG14210 antibody Fin15 antibody ICERE 1 antibody ICERE-1 antibody Inversely correlated with estrogen receptor expression 1 antibody Non-syndromic hearing impairment protein 5 antibody Nonsyndromic hearing impairment protein antibody |
| Product description | Non-syndromic hearing impairment protein 5 is a protein that in humans is encoded by the DFNA5 gene. Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. The observation that DFNA5 is epigenetically inactivated in a large number of cancers of frequent types (gastric, colorectal, and breast) is another important finding and is in line with its apoptosis-inducing properties. Indeed, if apoptosis is an intrinsic feature of DFNA5, shutting the gene down in tumor cells makes them more susceptible to uncontrolled cellular growth. Moreover, the fact that DFNA5 is regulated by P53 strongly suggests that DFNA5 is a tumor suppressor gene. |
| Immunogen | Synthetic peptide within human DFNA5 aa 30-230. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC-PFC |
| WB:1:500-1:2,000: ICC:1:50-1:100: IHC-P:1:50-1:200: FC:1:50-1:100: | |
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | 2310037D07Rik antibody 4932441K13Rik antibody Deafness autosomal dominant 5 antibody Deafness autosomal dominant 5 protein antibody DFNA5 antibody DFNA5 gene antibody DFNA5_HUMAN antibody Dfna5h antibody EG14210 antibody Fin15 antibody ICERE 1 antibody ICERE-1 antibody Inversely correlated with estrogen receptor expression 1 antibody Non-syndromic hearing impairment protein 5 antibody Nonsyndromic hearing impairment protein antibody |
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| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Cell membrane, cytosol. |
| SwissProt ID | O60443 |
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Application
Fig1: Western blot analysis of DFNA5/GSDME on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate
Application
Fig2: ICC staining of DFNA5/GSDME in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-DFNA5/GSDME antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-DFNA5/GSDME antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DFNA5/GSDME antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DFNA5/GSDME antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig7: Flow cytometric analysis of DFNA5/GSDME was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-48, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Hela cell lysates, HepG2 cell lysates, SiHa, rat testis tissue, human skin tissue, human breast carcinoma tissue, human placenta tissue, SH-SY5Y. |
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| Application Notes | WB:1:500-1:2,000: ICC:1:50-1:100: IHC-P:1:50-1:200: FC:1:50-1:100: |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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