| 存储条件 |
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| Product Name | CD10 [A1G4] |
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| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. |
| Immunogen | Synthetic peptide within human CD10 aa 200-300. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,IHC-P,FC |
| WB:1:500-1:2,000 IHC-P:1:200-1:1,000 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/mL |
| Purification | Unpurified |
| Alternative Names | Atriopeptidase antibody CALLA antibody CD10 antibody CD10 antigen antibody Common acute lymphocytic leukemia antigen antibody DKFZp686O16152 antibody EC 3.4.24.11 antibody Enkephalinase antibody EPN antibody Membrane metallo endopeptidase (neutral endopeptidase enkephalinase) antibody Membrane metallo endopeptidase (neutral endopeptidase enkephalinase CALLA CD10) antibody Membrane metallo endopeptidase antibody Membrane metallo endopeptidase variant 1 antibody Membrane metallo endopeptidase variant 2 antibody Membrane metalloendopeptidase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody Membrane metalloendopeptidase variant 1 antibody Membrane metalloendopeptidase variant 2 antibody MGC126681 antibody MGC126707 antibody MME antibody NEP antibody NEP_HUMAN antibody Neprilysin antibody neprilysin-390 antibody neprilysin-411 antibody Neutral endopeptidase 24.11 antibody Neutral endopeptidase antibody Neutral endopeptidase membrane-associated antibody SFE antibody Skin fibroblast elastase antibody |
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| Molecular Weight(MW) | 86 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P08473 |
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Application
Fig1: Western blot analysis of CD10 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-25, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-25, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-25, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig5: Flow cytometric analysis of CD10 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-25, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Daudi cell, human prostate tissue, human kidney tissue, human small intestine tissue, 296 cell. |
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| Application Notes | WB:1:500-1:2,000 IHC-P:1:200-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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