| 存储条件 |
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| Product Name | SERPINC1 [15F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANT3_HUMAN antibody Antithrombin antibody Antithrombin III antibody Antithrombin-III antibody AntithrombinIII antibody AT 3 antibody AT III antibody AT3 antibody AT3D antibody ATIII antibody Heparin cofactor I antibody MGC22579 antibody Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody Serine cysteine proteinase inhibitor clade C member 1 antibody Serine proteinase inhibitor clade C member 1 antibody Serpin C1 antibody Serpin family C member 1 antibody Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody SERPINC1 antibody THPH7 antibody |
| Product description | The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. |
| Immunogen | Synthetic peptide within human aa 100-400. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000: IHC-P:1:50-1:200: FC:1:50-1:100 : | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | ANT3_HUMAN antibody Antithrombin antibody Antithrombin III antibody Antithrombin-III antibody AntithrombinIII antibody AT 3 antibody AT III antibody AT3 antibody AT3D antibody ATIII antibody Heparin cofactor I antibody MGC22579 antibody Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody Serine cysteine proteinase inhibitor clade C member 1 antibody Serine proteinase inhibitor clade C member 1 antibody Serpin C1 antibody Serpin family C member 1 antibody Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody SERPINC1 antibody THPH7 antibody |
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| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Extracellular space or secreted. |
Application
Fig1: Western blot analysis of SERPINC1 on HL-60 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Western blot analysis of SERPINC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: SiHa cell lysate Lane 3: U937 cell lysate
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SERPINC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-SERPINC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SERPINC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-11, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-SERPINC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig7: Flow cytometric analysis of SERPINC1 was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-11, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | HL-60 cell, HepG2 cell, SiHa cell, U937 cell, rat testis tissue, human lung carcinoma tissue, human liver tissue, mouse kidney tissue. |
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| Application Notes | WB:1:500-1:2,000: IHC-P:1:50-1:200: FC:1:50-1:100 : |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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