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| Product Name | alpha 1 Antitrypsin [JF10-03] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | A1A antibody A1AT antibody A1AT_HUMAN antibody AAT antibody Alpha 1 antiproteinase antibody Alpha 1 antitrypsin antibody Alpha 1 antitrypsin null antibody Alpha 1 protease inhibitor antibody Alpha-1 protease inhibitor antibody Alpha-1-antiproteinase antibody alpha1 proteinase inhibitor antibody Alpha1AT antibody Dom1 antibody PI antibody PI1 antibody PRO2275 antibody Serine (or cysteine) proteinase inhibitor clade A member 1 antibody Serine protease inhibitor 1-1 antibody Serine protease inhibitor A1a antibody Serpin A1 antibody Serpin A1a antibody Serpin peptidase inhibitor clade A member 1 antibody Serpina1 antibody Short peptide from AAT antibody SPAAT antibody Spi1-1 antibody |
| Product description | Cumulative damage to lung tissue by Neutrophil Elastase is responsible for the development of pulmonary emphysema, an irreversible lung disease characterized by loss of lung elasticity. a 1-antitrypsin (AAT), a 394 amino acid hepatic acute phase protein, predominantly inhibits Neutrophil Elastase. AAT is highly expressed in liver and in cultured hepatoma cells and, to a lesser extent, in macrophages. AAT is a highly polymorphic glycosylated serum protein with characteristic isoelectric-focusing patterns for most variants. The gene encoding AAT maps to a region of human chromosome 14 that includes a related serine protease inhibitor (serpin) gene which encodes corticosteroid-binding globulin. Oxidation of the methionine 358 residue in the active center of AAT results in a dramatic decrease in inhibitory activity towards elastase. AAT also has a moderate affinity for plasmin and Thrombin. AAT deficiency is associated with a 20-30 fold increased risk of precocious pulmonary emphysema. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | A1A antibody A1AT antibody A1AT_HUMAN antibody AAT antibody Alpha 1 antiproteinase antibody Alpha 1 antitrypsin antibody Alpha 1 antitrypsin null antibody Alpha 1 protease inhibitor antibody Alpha-1 protease inhibitor antibody Alpha-1-antiproteinase antibody alpha1 proteinase inhibitor antibody Alpha1AT antibody Dom1 antibody PI antibody PI1 antibody PRO2275 antibody Serine (or cysteine) proteinase inhibitor clade A member 1 antibody Serine protease inhibitor 1-1 antibody Serine protease inhibitor A1a antibody Serpin A1 antibody Serpin A1a antibody Serpin peptidase inhibitor clade A member 1 antibody Serpina1 antibody Short peptide from AAT antibody SPAAT antibody Spi1-1 antibody |
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| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Secreted, Endoplasmic reticulum. |
| SwissProt ID | P01009 |
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Application
Fig1: Western blot analysis of alpha 1 Antitrypsin on human spleen lysates using anti-alpha 1 Antitrypsin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining alpha 1 Antitrypsin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining alpha 1 Antitrypsin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining alpha 1 Antitrypsin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin.| Positive Control | MCF-7, Hela, HepG2, human spleen tissue, human liver tissue, human breast carcinoma tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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