| 存储条件 |
|---|
| Product Name | S100P [JF95-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | S-100 proteins are small dimeric members of the EF-Hand superfamily that participate in moderating intracellular calcium signals by binding to and regulating specific proteins in a calcium-dependent manner. S-100P is a survival factor that is associated with different types of tumors and can bind and regulate effector proteins. R1881, a synthetic androgen, regulates S-100P expression. S-100P interacts with a receptor for advanced glycation end products (RAGE) and activates it, thereby increasing the rates of cell growth, division, migration and invasion. This suggests that S-100P acts in an auto-crine manner through RAGE to trigger cell proliferation and survival. S-100P may also positively affect anchorage-independent growth to improve tumor formation. S-100P monomers strongly interact with one another, but not with other S-100 polypeptides, suggesting that homodimer formation is necessary for S-100P to function. The S-100P dimers are then stabilized by hydrophobic contacts. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC,IP |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 IP:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | MIG9 antibody Migration inducing gene 9 antibody Protein S100-E antibody Protein S100-P antibody Protein S100P antibody S100 calcium binding protein P antibody S100 calcium-binding protein P antibody S100 P antibody S100E antibody S100P antibody S100P_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 10 kDa |
| Cellular Localization | Nucleus, Cytoplasm, Cell projection. |
| SwissProt ID | P25815 |
|---|
Application
Fig1: ICC staining S100P in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining S100P in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-S100P antibody. Counter stained with hematoxylin.| Positive Control | Hela, HepG2, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 IP:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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