| 存储条件 |
|---|
| Product Name | Calbindin [JF05-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The family of EF-hand type Ca2+-binding proteins includes Calbindin D28K, Calbindin D9K, S-100 α and β, Calgranulin A (also designated MRP8), Calgranulin B (also designated MRP14), Calgranulin C and the Parvalbumin family members, including Parvalbumin α and Parvalbumin β (also designated oncomodulin). Calbindin D28K, also known as calbindin, CALB1, D-28K or vitamin D-dependent calcium-binding protein, is a 261 amino acid protein with six EF-hand domains, four of which are active calcium-binding domains. Expressed in brain, ovary, uterus, testis, pancreas, liver, kidney and intestine, Calbindin D28K acts as a calcium-buffering agent and alters the activity of the plasma membrane ATPase. In neuronal cells, Calbindin D28K modulates calcium channel activity, calcium transients and intrinsic neuronal firing activity. Also, Calbindin D28K has been implicated to play a role in apoptosis and microtubule function. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | avian-type antibody CAB27 antibody CALB 1 antibody CALB antibody CALB1 antibody CALB1_HUMAN antibody Calbindin 1 28kDa antibody Calbindin antibody Calbindin D28 antibody D 28K antibody D-28K antibody D28K antibody OTTHUMP00000166027 antibody OTTHUMP00000225441 antibody RTVL H protein antibody Vitamin D dependent calcium binding protein antibody Vitamin D dependent calcium binding protein avian type antibody Vitamin D-dependent calcium-binding protein antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cytosol, Nucleus. |
Application
Fig1: Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain Lane 2: Mouse kidney
Application
Fig2: ICC staining Calbindin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Calbindin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.| Positive Control | PC-12, 293T, rat brain tissue, human kidney tissue, mouse kidney tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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