| 存储条件 |
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| Product Name | DFFA [2B10C8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Immunogen | Purified recombinant fragment of human DFFA (AA: 179-331) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,FC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | A330085O09Rik antibody Caspase activated deoxyribonuclease inhibitor short form antibody DFF 1 antibody DFF 45 antibody DFF alpha antibody DFF-45 antibody DFF1 antibody DFF35 antibody DFF45 antibody DFFA antibody Dffa DNA fragmentation factor alpha subunit antibody DFFA_HUMAN antibody DNA fragmentation factor 45 kDa subunit antibody DNA Fragmentation Factor Alpha Subunit antibody DNA fragmentation factor subunit alpha antibody DNA fragmentation factor 45 kD alpha subunit antibody DNA fragmentation factor 45kDa alpha polypeptide (DFFA) transcript variant 1 antibody DNA fragmentation factor 45kDa alpha polypeptide antibody DNA fragmentation factor alpha subunit antibody DNAation factor 45 kDa subunit antibody H13 antibody ICAD antibody ICAD L antibody ICAD S antibody Inhibitor of CAD antibody Inhibitor of Caspase Activated DNase antibody MGC143066 antibody OTTHUMP00000001903 antibody OTTHUMP00000001904 antibody RP23 121D17.3 antibody |
|---|---|
| Molecular Weight(MW) | 36.5kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | O00273 |
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Application
Western blot analysis of DFFA against human DFFA (AA: 179-331) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of DFFA against HEK293 (1) and DFFA (AA: 179-331)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of DFFA was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-32, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
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| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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