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| Product Name | gamma Tubulin [JF0972] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Gamma 1 tubulin antibody Gamma 2 tubulin antibody Gamma Tubulin 1 antibody Gamma Tubulin 2 antibody Gamma tubulin complex component 1 antibody Gamma-2-tubulin antibody GCP 1 antibody GCP1 antibody MGC131994 antibody TBG2_HUMAN antibody TUBG antibody TUBG1 antibody TUBG2 antibody TUBGCP1 antibody Tubulin gamma 1 chain antibody Tubulin gamma 2 chain antibody Tubulin gamma complex-associated protein 1 antibody Tubulin gamma-2 chain antibody tubulin, gamma 1 antibody tubulin, gamma 2 antibody tubulin, gamma polypeptide antibody Xgam antibody |
| Product description | Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRatMonkeyChickenHamsterCowDogFishXenopus |
| Concentration | 1mg/ml |
| Alternative Names | Gamma 1 tubulin antibody Gamma 2 tubulin antibody Gamma Tubulin 1 antibody Gamma Tubulin 2 antibody Gamma tubulin complex component 1 antibody Gamma-2-tubulin antibody GCP 1 antibody GCP1 antibody MGC131994 antibody TBG2_HUMAN antibody TUBG antibody TUBG1 antibody TUBG2 antibody TUBGCP1 antibody Tubulin gamma 1 chain antibody Tubulin gamma 2 chain antibody Tubulin gamma complex-associated protein 1 antibody Tubulin gamma-2 chain antibody tubulin gamma 1 antibody tubulin gamma 2 antibody tubulin gamma polypeptide antibody Xgam antibody |
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| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of gamma Tubulin on different lysates using anti-gamma Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Jurkat Lane 3: Mouse brain
Application
Fig2: ICC staining gamma Tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining gamma Tubulin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining gamma Tubulin in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Jurkat cells with gamma Tubulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, MCF-7, Hela, SHG-44, mouse testis tissue, mouse colon tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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