| 存储条件 |
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| Product Name | EDA2R [4A6B4] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | 9430060M22Rik antibody Ectodysplasin A2 isoform receptor antibody Ectodysplasin A2 receptor antibody EDA A2R antibody EDA-A2 receptor antibody EDA2R antibody EDAA2R antibody MGC124099 antibody OTTMUSP00000019574 antibody OTTMUSP00000019575 antibody RP23-188N15.1 antibody TNFRSF27 antibody TNR27_HUMAN antibody Tumor necrosis factor receptor superfamily member 27 antibody Tumor necrosis factor receptor superfamily member XEDAR antibody X linked ectodysplasin A2 receptor antibody X linked ectodysplasin receptor antibody X-linked ectodysplasin-A2 receptor antibody XEDAR antibody |
| Product description | X-linked ectodysplasin-A2 receptor (XEDAR) is a recently isolated member of the tumor necrosis factor receptor family that is highly expressed during embryonic development and binds to ectodysplatin-A2 (EDA-A2). Two predominantly expressed isoforms, XEDAR-s and XEDAR-L, differ by only a 21-amino region at the juxtamembrane region of the cytoplasmic domain. Neither isoform possesses a death domain and both have been shown to act mainly through TRAF3 and TRAF6 to activate the NF-kappa-B and JNK pathways. Cells transfected with XEDAR and treated with EDA-A2 cause the assembly of a secondary complex containing FADD, caspase-8 and caspase-10, leading to the activation caspase-8 and caspase-3, and finally apoptosis. The EDA-A2-induced apoptosis is dependent on caspase-9 activation, as various pharmacological and genetic inhibitors of caspase-8 blocked apoptosis following EDA-A2 treatment. |
| Immunogen | Purified recombinant fragment of human EDA2R (AA: extra 1-138) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | 9430060M22Rik antibody Ectodysplasin A2 isoform receptor antibody Ectodysplasin A2 receptor antibody EDA A2R antibody EDA-A2 receptor antibody EDA2R antibody EDAA2R antibody MGC124099 antibody OTTMUSP00000019574 antibody OTTMUSP00000019575 antibody RP23-188N15.1 antibody TNFRSF27 antibody TNR27_HUMAN antibody Tumor necrosis factor receptor superfamily member 27 antibody Tumor necrosis factor receptor superfamily member XEDAR antibody X linked ectodysplasin A2 receptor antibody X linked ectodysplasin receptor antibody X-linked ectodysplasin-A2 receptor antibody XEDAR antibody |
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| Molecular Weight(MW) | 32.8kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q9HAV5 |
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Application
Western blot analysis of EDA2R against human EDA2R (AA: extra 1-138) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EDA2R against HEK293 (1) and EDA2R (AA: extra 1-138)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of EDA2R was done on MOLT4 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-91, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | MOLT4 cells |
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| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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