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| Product Name | TIGIT [4C5B9] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp667A205 antibody ENSMUSG00000071552 antibody FLJ39873 antibody RGD1563191 antibody T cell immunoreceptor with Ig and ITIM domains antibody TIGIT antibody TIGIT_HUMAN antibody V-set and immunoglobulin domain containing 9 antibody V-set and immunoglobulin domain-containing protein 9 antibody V-set and transmembrane domain containing 3 antibody V-set and transmembrane domain-containing protein 3 antibody VSIG9 antibody VSTM3 antibody Washington University cell adhesion molecule antibody WUCAM antibody |
| Product description | TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is an immune receptor present on some T cells and Natural Killer Cells (NK). It is also identified as WUCAM and Vstm3. TIGIT could bind to CD155 (PVR) on dendritic cells (DCs), macrophages, etc. with high affinity, and also to CD112 (PVRL2) with lower affinity. Research has shown that TIGIT-Fc fusion protein could interact with PVR on dendritic cells and increase its IL-10 secretion level/decrease its IL-12 secretion level under LPS stimulation, and also inhibit T cell activation in vivo. TIGIT's inhibition of NK cytotoxicity can be blocked by antibodies against its interaction with PVR and the activity is directed through its ITIM domain. TIGIT and PD-1 has been shown to be over expressed on tumor antigen-specific (TA-specific) CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of TA-specific CD8+ T cells and TIL CD8+ T cells. It can be considered an immune checkpoint. Co-blockade of TIGIT and PD-1 pathways elicits tumor rejection in preclinical murine models. |
| Immunogen | Purified recombinant fragment of human TIGIT (AA: extra 22-141) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | DKFZp667A205 antibody ENSMUSG00000071552 antibody FLJ39873 antibody RGD1563191 antibody T cell immunoreceptor with Ig and ITIM domains antibody TIGIT antibody TIGIT_HUMAN antibody V-set and immunoglobulin domain containing 9 antibody V-set and immunoglobulin domain-containing protein 9 antibody V-set and transmembrane domain containing 3 antibody V-set and transmembrane domain-containing protein 3 antibody VSIG9 antibody VSTM3 antibody Washington University cell adhesion molecule antibody WUCAM antibody |
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| Molecular Weight(MW) | 26.3kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q495A1 |
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Application
Western blot analysis of TIGIT against human TIGIT (AA: extra 22-141) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-33 against HEK293 (1) and TIGIT (AA: extra 22-141)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of TIGIT was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-33, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | K562 cells |
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| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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