| 存储条件 |
|---|
| Product Name | TNFRSF6B [3C5H10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DcR3 antibody Decoy receptor 3 antibody Decoy receptor for Fas ligand antibody DJ583P15.1.1 antibody M68 antibody M68E antibody OTTHUMP00000031583 antibody TNF6B_HUMAN antibody TNFRSF6B antibody TR6 antibody Tumor necrosis factor receptor superfamily member 6B antibody Tumor necrosis factor receptor superfamily member 6b decoy antibody UNQ186/PRO212 antibody |
| Product description | This gene belongs to the tumor necrosis factor receptor superfamily. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death. It acts as a decoy receptor that competes with death receptors for ligand binding. Over-expression of this gene has been noted in gastrointestinal tract tumors. Read-through transcription into this gene from the neighboring upstream gene, which encodes regulator of telomere elongation helicase 1 (RTEL1), generates a non-coding transcript. |
| Immunogen | Purified recombinant fragment of human TNFRSF6B (AA: 30-300) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | DcR3 antibody Decoy receptor 3 antibody Decoy receptor for Fas ligand antibody DJ583P15.1.1 antibody M68 antibody M68E antibody OTTHUMP00000031583 antibody TNF6B_HUMAN antibody TNFRSF6B antibody TR6 antibody Tumor necrosis factor receptor superfamily member 6B antibody Tumor necrosis factor receptor superfamily member 6b decoy antibody UNQ186/PRO212 antibody |
|---|---|
| Molecular Weight(MW) | 32.7kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | O95407 |
|---|
Application
Western blot analysis of TNFRSF6B against human TNFRSF6B (AA: 30-300) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-10 against HEK293 (1) and TNFRSF6B (AA: 30-300)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of TNFRSF6B was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-10, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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