KIAA0474 antibody Rap1 GTPase activating protein 1 antibody RAP1 GTPase activating protein antibody Rap1 GTPase-activating protein 1 antibody RAP1GA1 antibody Rap1ga1 protein antibody Rap1GAP antibody Rap1GAP1 antibody RAP1GAPII antibody RAPGAP antibody RPGP1_HUMAN antibody
Product description
Rap1 GTPase activating protein (Rap1GAP) specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between Gα z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the α subunit, and also attenuates the ability of activated Gα z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to Gα z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
Immunogen
Recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG2b
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:50-1:100
Species Reactivity
Human
Concentration
2mg/ml
Target Information
Alternative Names
KIAA0474 antibody Rap1 GTPase activating protein 1 antibody RAP1 GTPase activating protein antibody Rap1 GTPase-activating protein 1 antibody RAP1GA1 antibody Rap1ga1 protein antibody Rap1GAP antibody Rap1GAP1 antibody RAP1GAPII antibody RAPGAP antibody RPGP1_HUMAN antibody
Fig1: Western blot analysis of RAP1GAP on human RAP1GAP recombinant protein using anti-RAP1GAP antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RAP1GAP on HEK293 (1) and RAP1GAP-hIgGFc transfected HEK293 (2) cell lysate using anti-RAP1GAP antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of A431 cells with RAP1GAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human RAP1GAP recombinant protein, RAP1GAP-hIgGFc transfected HEK293 cell lysate, A431.
Application Notes
WB:1:500-1:2,000 FC:1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
