For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilution
Western Blotting
1:1000
Simple Western™
1:250
Immunoprecipitation
1:50
Chromatin IP
1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-SMAD3 (Ser423/425) (C25A9) Rabbit Monoclonal Antibody detects endogenous levels of SMAD3 when phosphorylated at Ser423/425. This antibody does not cross-react with other family members.
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology:
Monkey, Xenopus, Zebrafish, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser423/425 of human SMAD3.
