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Mouse I-TAC ELISA Kit (CXCL11) is a Sandwich (quantitative) ELISA for the measurement of Mouse I-TAC (CXCL11) in Mouse in Biofluids, Cell Culture Media samples.
隐藏别名
Scyb11, Cxcl11, C-X-C motif chemokine 11, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, I-TAC
Colorimetric
Plasma, Cell culture supernatant, Serum
Mouse
Sandwich (quantitative)
< 6 pg/mL
5.487 - 4000 pg/mL
Microplate
Abcam's I-TAC Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse I-TAC in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse I-TAC coated on a 96-well plate. Standards and samples are pipetted into the wells and I-TAC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse I-TAC antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-TAC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Representative standard curve using ab100716

Representative standard curve using ab100716
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