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Mouse MCP1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse MCP1 in Mouse in Cell/Tissue Extracts samples.
隐藏别名
Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1
Colorimetric
Cell culture extracts, Tissue Extracts
Mouse
Sandwich (quantitative)
< 3 pg/mL
2.74 - 2000 pg/mL
Microplate
Abcam's MCP1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP1 in cell lysates and tissue lysates.
This assay employs an antibody specific for mouse MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisationAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Representative standard curve using ab100722
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