| 存储条件 |
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| Product Name | CD299 [8A1B3] |
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| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a transmembrane receptor and is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses, with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are common and have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 30835; often referred to as DC-SIGN or CD209). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants. |
| Immunogen | Purified recombinant fragment of human CD299 (AA: extra 237-399) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,FC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | C type lectin domain family 4 member M antibody C-type lectin domain family 4 member M antibody CD209 antigen like antibody CD209 antigen like protein 1 antibody CD209 antigen-like protein 1 antibody CD209L antibody CD209L1 antibody CD299 antibody CD299 antigen antibody CLC4M_HUMAN antibody CLEC4M antibody DC SIGN related protein antibody DC SIGN2 antibody DC SIGNR antibody DC-SIGN-related protein antibody DC-SIGN2 antibody DC-SIGNR antibody DCSIGN related protein antibody DCSIGNR antibody Dendritic cell-specific ICAM-3-grabbing non-integrin 2 antibody HP10347 antibody L SIGN antibody L-SIGN antibody Liver/lymph node specific ICAM3 grabbing nonintegrin antibody Liver/lymph node-specific ICAM-3-grabbing non-integrin antibody LSIGN antibody Mannose binding C type lectin DC SIGNR antibody MGC129964 antibody MGC47866 antibody |
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| Molecular Weight(MW) | 45.4kDa |
| Cellular Localization | Secreted and Cell membrane. |
| SwissProt ID | Q9H2X3 |
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Application
Western blot analysis of CD299 against human CD299 (AA: extra 237-399) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD299 against HEK293 (1) and CD299 (AA: extra 237-399)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD299 against L-02 (1), HepG2 (2), BEL-7402 (3), SMMC-7702 (4), and HL-7702 (5) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD299 was done on MOLT4 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-85, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | L-02, HepG2, BEL-7402, SMMC-7702, and HL-7702 cell lysate, MOLT4 cells |
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| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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