CD307c antibody Fc receptor homolog 3 antibody Fc receptor-like protein 3 antibody FcR-like protein 3 antibody FcRH3 antibody FcRL3 antibody FCRL3_HUMAN antibody hIFGP3 antibody IFGP family protein 3 antibody IFGP3 antibody Immune receptor translocation-associated protein 3 antibody Immunoglobulin superfamily receptor translocation associated protein 3 antibody IRTA3 antibody SH2 domain-containing phosphatase anchor protein 2 antibody SPAP2 antibody
Product description
This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains immunoreceptor-tyrosine activation motifs and immunoreceptor-tyrosine inhibitory motifs in its cytoplasmic domain and may play a role in regulation of the immune system. Mutations in this gene have been associated with rheumatoid arthritis, autoimmune thyroid disease, and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.
Immunogen
Purified recombinant fragment of human CD307C (AA: extra 18-153) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
CD307c antibody Fc receptor homolog 3 antibody Fc receptor-like protein 3 antibody FcR-like protein 3 antibody FcRH3 antibody FcRL3 antibody FCRL3_HUMAN antibody hIFGP3 antibody IFGP family protein 3 antibody IFGP3 antibody Immune receptor translocation-associated protein 3 antibody Immunoglobulin superfamily receptor translocation associated protein 3 antibody IRTA3 antibody SH2 domain-containing phosphatase anchor protein 2 antibody SPAP2 antibody
Western blot analysis of CD307C against human CD307C (AA: extra 18-153) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD307C against HEK293 (1) and CD307C (AA: extra 18-153)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD307C was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-77, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Positive Control
HL-60 cells
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
