| 存储条件 |
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| Product Name | MUC5B [7F5A1] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | 2300002I04Rik antibody A130042M24 antibody AV085033 antibody Cervical mucin antibody Cervical mucin MUC5B antibody High molecular weight salivary mucin MG1 antibody MG1 antibody MUC-5B antibody MUC5 antibody MUC5B antibody MUC5B_HUMAN antibody MUC9 antibody Mucin 5 subtype B, tracheobronchial antibody Mucin 5B antibody Mucin 5B oligomeric mucus/gel forming antibody Mucin-5 subtype B antibody Mucin-5B antibody Sublingual gland mucin antibody tracheobronchial antibody |
| Product description | This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. |
| Immunogen | Purified recombinant fragment of human MUC5B (AA: 26-166) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | 2300002I04Rik antibody A130042M24 antibody AV085033 antibody Cervical mucin antibody Cervical mucin MUC5B antibody High molecular weight salivary mucin MG1 antibody MG1 antibody MUC-5B antibody MUC5 antibody MUC5B antibody MUC5B_HUMAN antibody MUC9 antibody Mucin 5 subtype B tracheobronchial antibody Mucin 5B antibody Mucin 5B oligomeric mucus/gel forming antibody Mucin-5 subtype B antibody Mucin-5B antibody Sublingual gland mucin antibody tracheobronchial antibody |
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| Molecular Weight(MW) | 596kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q9HC84 |
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Application
Western blot analysis of MUC5B against human MUC5B (AA: 26-166) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of MUC5B against HEK293 (1) and MUC5B (AA: 26-166)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded rectum tissues using anti-MUC5B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1711-75, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of MUC5B was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-75, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells, rectum tissues |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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