Low-density lipoprotein receptor-related protein 1B LRP-1B Low-density lipoprotein receptor-related protein-deleted in tumor LRP-DIT LRP1B LRPDIT
Product description
This gene encodes a member of the low density lipoprotein (LDL) receptor family. These receptors play a wide variety of roles in normal cell function and development due to their interactions with multiple ligands. Disruption of this gene has been reported in several types of cancer.
Immunogen
Purified recombinant fragment of human LRP1B (AA: extra 25-161) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
Low-density lipoprotein receptor-related protein 1B LRP-1B Low-density lipoprotein receptor-related protein-deleted in tumor LRP-DIT LRP1B LRPDIT
Western blot analysis of LRP1B against human LRP1B (AA: extra 25-161) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of LRP1B against HEK293 (1) and LRP1B (AA: extra 25-161)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of LRP1B was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-69, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Positive Control
HL-60 cells
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
