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| Product Name | CD182 [5F7A11] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | C-X-C chemokine receptor type 2 antibody CD 182 antibody CD182 antibody CD182 antigen antibody CDw128b antibody Chemokine (CXC) receptor 2 antibody CMKAR2 antibody CXC-R2 antibody CXCR 2 antibody CXCR-2 antibody CXCR2 antibody CXCR2_HUMAN antibody GRO/MGSA receptor antibody High affinity interleukin-8 receptor B antibody IL 8 receptor type 2 antibody IL 8R B antibody IL-8 receptor type 2 antibody IL-8R B antibody IL8 RB antibody IL8 receptor type 2 antibody IL8R B antibody IL8R2 antibody IL8RA antibody Interleukin 8 Receptor B antibody Interleukin 8 receptor, beta antibody Interleukin 8 receptor, type 2 antibody |
| Product description | The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. |
| Immunogen | Purified recombinant fragment of human CD182 expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | C-X-C chemokine receptor type 2 antibody CD 182 antibody CD182 antibody CD182 antigen antibody CDw128b antibody Chemokine (CXC) receptor 2 antibody CMKAR2 antibody CXC-R2 antibody CXCR 2 antibody CXCR-2 antibody CXCR2 antibody CXCR2_HUMAN antibody GRO/MGSA receptor antibody High affinity interleukin-8 receptor B antibody IL 8 receptor type 2 antibody IL 8R B antibody IL-8 receptor type 2 antibody IL-8R B antibody IL8 RB antibody IL8 receptor type 2 antibody IL8R B antibody IL8R2 antibody IL8RA antibody Interleukin 8 Receptor B antibody Interleukin 8 receptor beta antibody Interleukin 8 receptor type 2 antibody |
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| Molecular Weight(MW) | 40.8kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P25025 |
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Application
Western blot analysis of CD182 against human CD182 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-55 against HEK293 (1) and CD182-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of CD182 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1711-55, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CD182 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1711-55, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD182 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-55, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells HL-60 cells cervical cancer |
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| Application Notes | WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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