Cd210 antibody CDw210b antibody CRF2 4 antibody CRF2-4 antibody CRFB4 antibody Cytokine receptor class II antibody Cytokine receptor class II CRF2 4 antibody Cytokine receptor class-II member 4 antibody Cytokine receptor family 2 member 4 antibody Cytokine receptor family II member 4 antibody D21S58 antibody D21S66 antibody I10R2_HUMAN antibody IL-10 receptor subunit beta antibody IL-10R subunit 2 antibody IL-10R subunit beta antibody IL-10R2 antibody IL-10RB antibody IL10R2 antibody IL10RB antibody Interleukin 10 receptor beta antibody Interleukin-10 receptor subunit 2 antibody Interleukin-10 receptor subunit beta antibody
Product description
The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.
Immunogen
Purified recombinant fragment of human IL10RB (AA: extra 20-220) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WB
WB:1:500-1:2,000
Species Reactivity
HumanRat
Concentration
1mg/mL
Target Information
Alternative Names
Cd210 antibody CDw210b antibody CRF2 4 antibody CRF2-4 antibody CRFB4 antibody Cytokine receptor class II antibody Cytokine receptor class II CRF2 4 antibody Cytokine receptor class-II member 4 antibody Cytokine receptor family 2 member 4 antibody Cytokine receptor family II member 4 antibody D21S58 antibody D21S66 antibody I10R2_HUMAN antibody IL-10 receptor subunit beta antibody IL-10R subunit 2 antibody IL-10R subunit beta antibody IL-10R2 antibody IL-10RB antibody IL10R2 antibody IL10RB antibody Interleukin 10 receptor beta antibody Interleukin-10 receptor subunit 2 antibody Interleukin-10 receptor subunit beta antibody
Western blot analysis of IL10RB against human IL10RB (AA: extra 20-220) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-51 against HEK293 (1) and IL10RB (AA: extra 20-220)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-51 against Hela (1), Jurkat (2), Ramos (3), Raji (4), and C6 (5) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of IL10RB was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-51, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Positive Control
Hela, Jurkat, Ramos, Raji, and C6 cell HL-60 cells
Application Notes
WB:1:500-1:2,000
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
