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| Product Name | CD97 [6D7E6] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | ADGRE5 antibody Adhesion G protein-coupled receptor E5 antibody CD 97 antibody CD_antigen=CD97 antibody CD97 antibody CD97 antigen antibody CD97 antigen subunit beta antibody CD97 molecule antibody CD97_HUMAN antibody Leukocyte antigen CD97 antibody Seven span transmembrane protein antibody Seven transmembrane helix receptor antibody Seven transmembrane heterodimeric receptor associated with inflammation antibody TM7LN 1 antibody TM7LN1 antibody |
| Product description | This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. |
| Immunogen | Purified recombinant fragment of human CD97 (AA: extra 419-552) expressed in E. Coli. |
| Clonality | Monoclonal |
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| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | ADGRE5 antibody Adhesion G protein-coupled receptor E5 antibody CD 97 antibody CD_antigen=CD97 antibody CD97 antibody CD97 antigen antibody CD97 antigen subunit beta antibody CD97 molecule antibody CD97_HUMAN antibody Leukocyte antigen CD97 antibody Seven span transmembrane protein antibody Seven transmembrane helix receptor antibody Seven transmembrane heterodimeric receptor associated with inflammation antibody TM7LN 1 antibody TM7LN1 antibody |
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| Molecular Weight(MW) | 91.8kDa |
| Cellular Localization | Cell membrane and Secreted. |
| SwissProt ID | P48960 |
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Application
Western blot analysis of CD97 against human CD97 (AA: extra 419-552) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-48 against HEK293 (1) and CD97 (AA: extra 419-552)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CD97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1711-48, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-CD97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1711-48, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD97 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-48, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells rectum cancer endometrial cancer |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
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